Composite

Part:BBa_K3237028:Design

Designed by: Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-16)


nuiA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

not codon optimized. No changes to the sequence was done as the implementation of this gene would have been for it to be placed within the genome. Is a part of a kill switch designed by Lethbridge iGEM 2019. Terminator used was BBa_B0015 as used in the literature [1].


Source

Genbank X77568.1 Anabaena sp. nuiA gene

References

1.Čelešnik, H., Tanšek, A., Tahirović, A., Vižintin, A,. Mustar, J., Vidmar, V., and Dolinar, M. (2016) Biosafety of biotechnologically important microalgae: intrinsicsuicide switch implementation in cyanobacteriumSynechocystissp.PCC 6803. The Company of Biologists. 5, 519-528.